解剖学报

›› 2009, Vol. 40 ›› Issue (3): 446-450.doi: 10.3969/j.issn.0529-1356.2009.03.021

• 论著 • 上一篇    下一篇

重组人内抑素对佐剂性关节炎大鼠滑膜组织p21、cyclin D1及CDK4表达的影响

黄学应1,2; 陈飞虎1* ; 张晓明2 ; 刘永靖1,3; 袁凤来4   

  1. 1. 安徽医科大学药学院，合肥 230032； 2.安徽医科大学人体解剖学教研室，合肥 230032；3.中国人民解放军105医院胸心外科，合肥 230031； 4.无锡市第三人民医院，江苏 无锡 214041
  • 收稿日期:2008-11-09 修回日期:2008-12-16 出版日期:2009-06-06
  • 通讯作者: 陈飞虎

Effects of recombinant human endostatin on the expression of p21, cyclin D1 and CDK4 in synovial tissue in rats with adjuvant arthritis

  1. 1.College of Pharmacy,Anhui Medical University, Hefei 230032, China;2.Department of Anatomy, Anhui Medical University, Hefei 230032, China；3.Department of Cardiothoracic Surgery,the 105th Hospital of PLA, Hefei 230031, China;4.The Third People’s Hospital of Wuxi, Jiangsu Wuxi 214041, China
  • Received:2008-11-09 Revised:2008-12-16 Online:2009-06-06
  • Contact: CHEN Fei-hu1

关键词: 内抑素, 佐剂性关节炎, p21, cyclin D1, CDK4, 实时荧光定量PCR, 免疫印迹法, 大鼠

Abstract: Objective To observe the effect of recombinant human endostatin(rhEndostatin) on the expression of p21,cyclin D1 and CDK4 genes in synovial tissue in rats with adjuvant arthritis(AA) and explore molecular mechanisms of the inhibitory effect of rhEndostatin on proliferation of fibroblast-like synoviocytes(FLS) in AA rats. Methods Thirty-six male SD rats were used in the study. They were divided at random into normal, AA model and rhEndostatin (2.5mg/kg)treated groups. Twelve rats were taken from each group. Adjuvant arthritis was induced in rats in AA model and rhEndostatin(2.5mg/kg)treated group. The effect of rhEndostatin on the expression of p21,cyclin D1,CDK4 mRNA and cyclin D1 protein in synovial tissue in AA rats was examined quantitatively by realtime fluorescent quantitative PCR and Western blotting assays,respectively. Results The expression levels of p21, cyclinD1 mRNA and cyclinD1 protein were significantly decreased by rhEndostatin, compared with the levels in AA synovial tissues (P<0.01). However, CDK4 mRNA expression levels in synovial tissue treated with rhEndostatin were significantly higher than those in AA control samples (P<0.01). Conclusion Recombinant human endostatin decreases cyclinD1 expression levels in AA synovial tissues, which may be one of the molecular mechanisms of the inhibitory effect of rhEndostatin on AA FLS pro

Key words: Endostatin, Adjuvant arthritis, p21, cyclin D1, CDK4, Realtime fluorescent quantitative PCR, Western blotting, Rat

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